제품소개
Molecular Biology Instruments – Gene Transduction
Gene transduction (Gene gun)
Gene transduction (Gene gun)
Gene gun technology (particle bombardment) is a new gene introduction technique that accelerates micron-sized gold or tungsten particles encapsulated with nucleic acids to the desired speed into cells, tissues or organs. It can be used to obtain instantaneous, stable and efficient expression and transformation. It is fast, easy, safe and efficient.
Features
Simple, fast, flexible and suitable for a wide range of cell types
Facilitates transient and stable expression
Requires only a small amount of DNA and cells, no vector DNA
Can be commonly introduced without restrictions for large fragments of DNA plasmids
In vivo and in vitro transformation and high specificity
Unique circular transformation of the bombardment chamber, which is removable for easy cleaning and sterilization
Low cost of material use, costing $18 per gun in addition to high-pressure gas
High reproducibility
Uniform particle distribution
Maximum control of the experimental process
System Standard Configuration
Host , vacuum pump, 1000 gun dissipation (3.5/4.5/7Mpa ruptured membrane, carrier film, stainless steel mat, 2 bottles of gold powder , 1 bottle of tungsten powder )
Application Areas
Animals: tissues (skin, organs), cells, organs, etc.
Plants: living plants, leaves, or other plant tissues
Yeast, bacteria, and other microorganisms
Gene expression research
Agricultural biotechnology and crop improvement
Plants vaccines
Main Technical Parameters
| Target region | (big)40cm2 |
| Pressure range | 3-12MPa |
| Target Type | Animals: cell and organ culture Plants: The smaller of the whole plants, cell culture, explant, yeast, |
| bacteria and other micro-organelles, chloroplast, mitochondria | |
| Voltage | 220V/110V |
| Gas | Helium or nitrogen (Helium is better) |
| Metal Particle | Φ0.8-1.5μm; Φ1.5-3.0μm |
The Handheld Gene Gun is a convenient handheld device. It allows for rapid and direct in situ gene transfer to cells. The system uses adjustable helium or nitrogen pressure to drive gold particles, pre-packaged with DNA, RNA or other biological material, located on the inside of a small plastic tube, directly into the cell, making transgenic work more flexible to perform.
Features
Highly targeted: no extraneous genes or proteins are introduced into the sample
Convenient and fast: portable design allows transformation to be performed in the field
Highly functional: enables transformation of cells with specific growth needs compared to other methods
Low sample requirements: only small amounts of DNA and cells are required, no vector DNA required, and no restrictions on large fragments of DNA
High transformation rate: small shooting area and uniform particle distribution allow transformation of different sizes of receptors, ensuring transformation efficiency
Facilitates transient and stable expression
Application Areas
Animals: any exposure to gene gun outside the tissue (skin and organs), cell, organ, etc
Plants: the field and greenhouse plants explant
Yeast, bacteria and other microorganisms
Gene expression studies
Agricultural biotechnology and crop improvement
Plant vaccine
Organelles, chloroplasts and mitochondria
Main Technical Parameters
| Experimental conditions | In situ, in vitro, in vivo, from the body |
| Target region | （small）2cm2 |
| Pressure range | 0.5-5MPa (12 times per magazine magazine rotation) |
| Target type | Animals: any organization (skin,organs) exposure to gene gun；cells, explant and organ culture Plants: Field and greenhouse use, plant cell culture and explant, yeast, bacteria, other microbes |
| Voltage | 9V (capable of firing 1000 times) |
| Gas | Helium or nitrogen |
| Metal particel Bombardment way | Φ0.8-1.5μm/Φ1.5-3.0μm Magazine sample points directly or nylon |
